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Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing - Nature Communications

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By Basheer Becerra, Ana P. Kutschat, Justin Delano, Eric Che, Ting Wu, Sophie Muller, Madelynn N. Whittaker, Manfred Lehner, Michael Love, Benjamin P. Kleinstiver, Martin Jankowiak, Daniel Bauer, Davide Seruggia, Luca Pinello, Sandra Wittibschlager, Anzhelika Tauber, Marlena Starrs, Christina S. Horstmann, Elise Sylvander
| Nature Verified
Abstract CRISPR tiling screens have enabled the characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing and enrichment analysis. This study introduces an end-to-end experimental assay and computational pipeline, which leverages targeted sequencing of CRISPR-introduced alleles at the endogenous target locus following dense base-editing mutagenesis.
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