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Eric Stanley Pringle on Muck Rack

Eric Stanley Pringle

As seen in: BioRxiv
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A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNA

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By Brett A. Duguay, John Rohde, Trinity H Tooley, Eric Stanley Pringle
| BioRxiv
Abstract Coronaviruses have large, positive-sense single-stranded RNA genomes that challenge conventional strategies for mutagenesis. Here, we report the development of a new reverse genetics system for the endemic human coronavirus (HCoV) OC43 that utilizes transformation-associated recombination (TAR) to assemble complete viral genomes from dsDNA genome fragments via homologous recombination in Saccharomyces cerevisiae.
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