Evan E. Hubbard

1 Introduction The lens is a unique component of the human eye that focuses light into the retina for proper detection of visual stimuli. The bulk of the lens is made up of fiber cells. When these cells mature, organelles are destroyed, leaving behind a highly concentrated proteinaceous matrix that allows unimpeded transmission of light (Augusteyn and Stevens 1998).

Introduction A frequently mentioned advantage of data-independent acquisition (DIA) proteomics approaches is the potential capture of information not originally sought when then data were acquired. This advantage is anticipated because DIA experiments are designed to collect information about all species eluting from chromatographic separation without selection as occurs in data-dependent acquisition (DDA). Despite the potential for reanalysis of DIA data, examples are limited.