Evelyn L. Eastwood on Muck Rack

Evelyn L. Eastwood

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MCM double hexamer loading visualized with human proteins - Nature

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By Florian Weissmann, Julia F. Greiwe, Thomas Pühringer, Emma C. Couves, Evelyn L. Eastwood
| Nature
Abstract Eukaryotic DNA replication begins with the loading of the MCM replicative DNA helicase as a head-to-head double hexamer at origins of DNA replication1,2,3. Our current understanding of how the double hexamer is assembled by the origin recognition complex (ORC), CDC6 and CDT1 comes mostly from budding yeast. Here we characterize human double hexamer (hDH) loading using biochemical reconstitution and cryo-electron microscopy with purified proteins.
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