Fabian Link on Muck Rack

Fabian Link

As seen in: Nature, Wiley Online Library, MDPI, eLife
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Articles

Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editor

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By Nicole May, Anh B. Cao, Annika Schmid, Fabian Link
| eLife
eLife Assessment This important article describes a meticulously-developed improved strategy for generation of functionally null mutants in Leishmania spp. via cytosine base editing, with reduced background toxicity and enhanced efficiency relative to a previously-described method. The authors show use of the strategy in a small-scale loss-of-function screen, providing compelling evidence that large-scale screens will be possible.
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Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA Polymerase, and a cytosine base editor

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By Nicole May, Annika Schmid, Fabian Link, Ngoc Anh Cao
| biorxiv.org
Abstract The ability to analyse the function of all genes in a genome is highly desirable, yet challenging in Leishmania due to a repetitive genome, limited DNA repair mechanisms and lack of RNA interference in most species.
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The actomyosin system is essential for the integrity of the endosomal system in bloodstream form Trypanosoma brucei

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By Fabian Link, Sisco Jung, Xenia Malzer, Felix Zierhut
| eLife
eLife assessment This important study builds on a previous publication, demonstrating that T. brucei has a continuous endomembrane system, which probably facilitates high rates of endocytosis. Using a range of cutting-edge approaches, the authors present compelling evidence that an actomyosin system, with the myosin TbMyo1 as an active molecular motor, is localized close to and can associate with the endosomal system in the bloodstream form of T. brucei.
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