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Felipe Velasquez Moros on Muck Rack

Felipe Velasquez Moros

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Articles

Abundance-biased codon diversification prevents recombination in AAV production and ensures robust in vivo expression of functional FRET sensors - Communications Biology

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By Lazaros Vasilikos, Henri S. Zanker, Tomer Kagan, Tal Laviv, Michael Arand, Aiman S. Saab, Bruno Weber, Jan Dernic, Afroditi Eleftheriou, Rachel M. Meister, Felipe Velasquez Moros
| Nature Verified
Abstract The delivery of genetically encoded fluorescent sensors via adeno-associated viral vectors (AAVs) enables the quantification of biological analytes with high spatiotemporal resolution in living animals. In this study, we expose an unreported problem of the approach, in which the presence of repeated subsequences in the sensor’s DNA sequence triggers recombination during AAV production.
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