Genotyping and genetic analysis DNA was extracted from each RIL of the two populations and their parents following the CTAB method described by Hulbert & Bennetzen (1991). The straighthead-linked markers, namely, RM282, RM225, RM2, AP3858-1, and RM206, were used to screen the RILs of the two populations. DNA amplification was performed as previously described (Pan et al., 2012). As to genotyping, alleles corresponding to resistant or susceptible parents were noted as “a” or “b,” respectively.
