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Franz Ake on Muck Rack

Franz Ake

As seen in: BioRxiv
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Articles

Quantification of transcript isoforms at the single-cell level using SCALPEL

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By Marcel Schilling, Akshay ganesh, Franz Ake, Sandra Maria Fernandez-Moya
| BioRxiv
Abstract Single-cell RNA sequencing (scRNA-seq) has facilitated the study of gene expression and the development of new tools to quantify transcript in individual cells. Yet, most of these methods have low sensitivity and accuracy. Here we present SCALPEL, a Nextflow-based tool to quantify and characterize transcript isoforms at the single-cell level using standard 3′ based scRNA-seq data. SCALPEL predictions have higher sensitivity than other tools and can be validated experimentally.
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