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Gaoao Liu

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SiCLAT: simultaneous imaging of chromatin loops and active transcription in living cells - Genome Biology

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By Xin Wan, Jie Kong, Xiaodi Hu, Lulu Liu, Yuanping Yang, Hu Li, Fengling Chen, Dan Zhang, DaHai Zhu, Yong Zhang, Gaoao Liu, Xingchen Niu
| Genome Biology Verified
SiCLAT (CAG-LSL-dCas9-P2A-dCas13d-polyA) transgenic mice in the C57BL/6j background were generated by the Model Animal Research Center of Nanjing University. Mice were housed in a pathogen-free facility and given free access to water and standard rodent chow under the following conditions: 21 °C ambient temperature, 50–60% humidity, and 12 h dark/light cycle. All animal procedures were approved by the Animal Ethics Committee of Peking Union Medical College, Beijing, China (ACUC-A01-2016–003).
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