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Giulia Viola

As seen in: BioRxiv, Cell Press
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Articles by Giulia Viola

Doublecortin reinforces microtubules to promote growth cone advance in soft environments

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By Alessandro Dema, Rabab Charafeddine, Jeffrey van Haren, Shima Rahgozar, Giulia Viola, Kyle Jacobs, Matthew L. Kutys, Torsten Wittmann, Kyle Jacobs
| Cell Press Verified
Keywords neuron morphogenesis growth cone doublecortin lissencephaly cytoskeleton microtubule traction force microscopy genome editing human-induced pluripotent stem cells biomechanics Results and discussion Because most pathogenic doublecortin (DCX) missense mutations occur in one of the two conserved DCX domains,2,9,10,11,12 we expressed EGFP-tagged DCX carrying such mutations in non-neuronal retinal pigment epithelial (RPE) cells and measured microtubule (MT) binding in cells expressing...
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Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

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By Kyle Jacobs, Joël Lemière, Matthew L. Kutys, Giulia Viola
| BioRxiv
Abstract To standardize comparison of fluorescent proteins and independently determine which monomeric StayGold variant is best for live microscopy, we analyzed fluorescent protein tagged I3-01 peptides that self-assemble into stable sixty subunit dodecahedrons inside live cells. We find mStayGold is 3-fold brighter and 3-fold more photostable compared with EGFP and superior to other monomeric variants in mammalian cytoplasm.
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