Gucan Dai

Abstract Voltage sensors are crucial for the electromechanical coupling of rapid delayed rectifier hERG channels in cardiac pacemaking. They detect and respond to changes in membrane voltage by traversing across the membrane electric field. Mutations in voltage-sensing arginines of hERG associated with Long QT syndrome induce distinct channel gating behaviors, yet knowledge about voltage sensing in these mutants remains limited.

Abstract Ion channels function within a membrane environment characterized by dynamic lipid compartmentalization. Limited knowledge exists regarding the response of voltage-gated ion channels to transmembrane potential within distinct membrane compartments. By leveraging fluorescence lifetime imaging microscopy (FLIM) and Förster resonance energy transfer (FRET), we visualized the localization of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in membrane domains.

Abstract Ion channels function within a membrane environment characterized by dynamic lipid compartmentalization. Limited knowledge exists regarding the response of voltage-gated ion channels to transmembrane potential within distinct membrane compartments. By leveraging fluorescence lifetime imaging microscopy (FLIM) and Förster resonance energy transfer (FRET), we visualized the localization of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in membrane domains.