Guoan Yin

1 Introduction Permafrost is ground that remains at or below 0°C for at least two consecutive years, covering 22% of the Earth's exposed land surface in Northern Hemisphere (Obu et al., 2019). Due to its sensitivity to climate change, permafrost is warming and thawing on a global scale, with ground temperature increasing by 0.29 ± 0.12°C in recent decades (Biskaborn et al., 2019).

2. Materials and Methods 2.1. Animals and General Procedures All experiments were approved by and conducted according to the guidelines of the Science and Technology Ethics Committee of the Heilongjiang Bayi Agricultural University (ethics code: DWKJXY20230045). This study used Roman-white laying hen chicks as the experimental animals.