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CD74-Downregulation of Placental Macrophage-Trophoblastic Interactions in Preeclampsia

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By Herbert Schulz, Friedrich C. Luft, Dominik Müller, Lukasz Przybyl, Nadine Haase, Michaela Golic, Julianna Rugor, Maria Emilia Solano, Petra Clara Arck, Martin Gauster, Berthold Huppertz, Christoph Emontzpohl, Christian Stoppe, Jürgen Bernhagen, Lin Leng, Richard Bucala, Arnd Heuser, M. Susanne Weedon-Fekjær, Guro M. Johnsen, Dirk Peetz, Ralf Dechend
| circres.ahajournals.org
Methods and Results: We performed whole-genome expression analysis of placental tissue. CD74 was one of the most downregulated genes in placentas from preeclamptic women. By reverse transcriptase-polymerase chain reaction, we confirmed this finding in early-onset (<34 gestational week, n=26) and late-onset (≥34 gestational week, n=24) samples from preeclamptic women, compared with healthy pregnant controls (n=28). CD74 protein levels were analyzed by Western blot and flow cytometry.
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