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• The present study investigated whether day 3 blastomere number has an effect on the clinical outcomes during single vitrified-warmed blastocyst transfer cycles. • A total of 3294 vitrified-warmed single day 5 blastocyst transferred cycles were divided into ≥ 7 and < 7 blastomere groups depending on the day 3 embryo blastomere number.
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