Han-Shin Kim

PLOS ONE, Aug 2023 Although detection of gram-negative bacteria (GNB) in body fluids is important for clinical purpose, traditional gram staining and other recently developed methods have inherent limitations in terms of accuracy, sensitivity, and convenience. To overcome the weakness, this study proposed a method detecting GNB based on specific binding of polymyxin B (PMB) to lipopolysaccharides (LPS) of GNB.

Loading metrics Open Access Peer-reviewed Research Article Citation: Kang H-J, Lee S-H, Kim H-S, Jung YW, Park H-D (2023) Rapid and sensitive detection of gram-negative bacteria using surface-immobilized polymyxin B. PLoS ONE 18(8): e0290579. https://doi.org/10.1371/journal.pone.0290579 Editor: Ivone Vaz-Moreira, Universidade Catolica Portuguesa, PORTUGAL Received: April 10, 2023; Accepted: August 11, 2023; Published: August 28, 2023 Copyright: © 2023 Kang et al.

1. Introduction Antimicrobial resistance (AMR) poses a major threat to human health around the world (Antimicrobial Resistance Collaborators, 2022). Recent study reported that 1.27 million deaths globally in 2019 were directly attributed to resistant bacteria and a further 4.9 million deaths were associated with bacterial AMR (Antimicrobial Resistance Collaborators, 2022).