Hesung Now

Correction to: Scientific Reportshttps://doi.org/10.1038/s41598-017-14726-w, published online 03 November 2017 In Figure 6b, data from MEF cells were mistakenly inserted in place of data from A549/HeLa cells. The correct Figure 6b appears as Figure 1. The conclusions are not affected by this change. Figure 1 (b) After patterning heterotypic A549 and HeLa cells according to the designs shown in (a), cells were incubated for 48 h and infected with influenza virus (10 TCID50 mL−1) for 24 h.

We inkjet-printed cell-laden ink onto predestined locations of a cell culture dish filled with cell culture medium (Fig. 1a). Complex cell patterns were fabricated using a computer-controlled printing system with a motorized x-y stage and a z-axis elevating nozzle. As ink, living cells were suspended in cell culture medium at a density of 6 × 106 cells mL−1. The shear viscosity of the cell-laden ink was evaluated before printing (Supplementary Fig. 1).

A population often contains distinct sub-populations, thereby increasing the complexity of the overall heterogeneity. However, the cellular origin and biological relevance of sub-populations in cell population have not been clearly identified. Here we demonstrated the novel roles of ISGylation, which is an IFN-induced post-translational modification, controlling heterogeneity at the population level in cultured adherent cells.