Ho Hang Leung

Abstract Mitochondria support the energetic demands of the cells. Autophagic turnover of mitochondria serves as a critical pathway for mitochondrial homeostasis. It is unclear how bioenergetics and autophagy are functionally connected. Here, we identify an endolysosomal membrane protein that facilitates autophagy to regulate ATP production in glia. We determined that Drosophila tweety (tty) is highly expressed in glia and localized to endolysosomes.

1 INTRODUCTION The Tweety homologs (TTYHs) are evolutionarily conserved membrane proteins in multicellular eukaryotes. The founding member was identified in the flightless genomic locus of Drosophila melanogaster and was named tweety (tty) (Campbell et al., 2000). Humans possess three paralogs, namely TTYH1, TTYH2, and TTYH3.

3. Materials and Methods 3.1. Reagents Dulbecco’s Modified Eagle medium (DMEM), penicillin–streptomycin, Hank’s Balanced Salt Solution (HBSS), tris(hydroxymethyl)-1,3-propanediol hydrochloride (TRIS-HCl), and trypsin were purchased from Gibco-Invitrogen (Carlsbad, CA, USA).