Hongcang Gu

Abstract The integration of DNA methylation and transcriptional state within single cells is of broad interest. Several single-cell dual- and multi-omics approaches have been reported that enable further investigation into cellular heterogeneity, including the discovery and in-depth study of rare cell populations. Such analyses will continue to provide important mechanistic insights into the regulatory consequences of epigenetic modifications.

Abstract Genetic and epigenetic intra-tumoral heterogeneity cooperate to shape the evolutionary course of cancer1. Chronic lymphocytic leukaemia (CLL) is a highly informative model for cancer evolution as it undergoes substantial genetic diversification and evolution after therapy2,3. The CLL epigenome is also an important disease-defining feature4,5, and growing populations of cells in CLL diversify by stochastic changes in DNA methylation known as epimutations6.

a) Individual cells are binned into M-G , G -S, S and G -M based on expression of known cell cycle regulated genes (Kowalczyk, M.S. et al. Genome Res 25, 1860–72, 2015). Of the 30,311 genes that were ranked according to their differential regulation throughout G -S and S-phase, only 1,085 show notable dynamics (Supplementary Data Set 2; Methods, FDR < 0.05).