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Huey-Yee Lim

As seen in: BioRxiv
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Articles

Designing Efficient Enzymes: Eight Predicted Mutations Convert a Hydroxynitrile Lyase into an Efficient Esterase

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By Guillem Casadevall, Colin Pierce, Javier Iglesias-Fernández, Huey-Yee Lim
| BioRxiv
Abstract Hydroxynitrile lyase from rubber tree (HbHNL) shares 45% identical amino acid residues with the homologous esterase from tobacco, SABP2, but the two enzymes catalyze different reactions. The x-ray structures reveal a serine-histidine-aspartate catalytic triad in both enzymes along with several differing amino acid residues within the active site.
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