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Polyphyllin II (PPII) Enhances the Sensitivity of Multidrug-resistant A549/DDP Cells to Cisplatin by Modulating Mitochondrial Energy Metabolism
Materials and Methods Cell lines and cell culture. A549 and A549/DDP cells were procured from Guangzhou Geniou Biotechnology Co., Ltd (Guangzhou, PR China) and maintained in DMEM media supplemented with 10% fetal bovine serum. These cells were cultured in a cell incubator at 37°C with 5% CO2 and maximum humidity, with regular passaging. The final concentration of DDP in A549/DDP cells was 2.1:0.002 mg/ml. Chemicals and drug preparations.
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