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Inhibition of the E3 ligase UBR5 stabilizes TERT and protects vascular organoids from oxidative stress - Journal of Translational Medicine
One line of hiPSCs was obtained from Nuwacell (RC01001-B) and cultured in complete TeSR™-E8™ medium (Stemcell, 05990). HUVECs (Sciencell, 8000) were cultured in endothelial cell medium (Sciencell, 1001). HEK293T cells were acquired from the American Type Culture Collection (Maryland, USA) and grown in Dulbecco's modified Eagle medium (Macgene, CM10013) supplemented with 10% fetal bovine serum (Excell Bio, FND500). All cells were incubated at 37 °C in a humidified atmosphere with 5% CO2.
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