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Articles

FACS-based detection of extracellular ASC specks from NLRP3 inflammasomes in inflammatory diseases

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By Musculoskeletal Medicine, Joanne Topping, ImmunAid Consortium
| Oxford Academic Verified
The apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) is crucial for inflammasome assembly and activation of several inflammasomes, including NLRP3. ASC aggregates are detected in human sera post pyroptotic cell death, but their inflammasome origin remains unclear. This study aimed to develop a method to detect ASC aggregates originating from NLRP3 inflammasomes.
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