Inoka Twickler

Materials and methods Research-grade UCARTCS1 cells and control cells were generated by Cellectis SA (Paris, France). UCARTCS1 cells are gene-edited T-cells generated from normal healthy donor peripheral blood T-cells. Upon initial stimulation with anti-CD3/CD28 reagents, activated T-cells were electroporated with mRNAs encoding TALEN to inactivate the TCRα constant chain (TRAC) gene in order to disrupt surface expression of TCRαβ and to minimize the risk of GvHD.