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iPSC Laboratory

As seen in: Biomed Central (BMC), Stem Cell Reports
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Articles

Proteomic analysis across patient iPSC-based models and human post-mortem hippocampal tissue reveals early cellular dysfunction and progression of Alzheimer’s disease pathogenesis | Acta Neuropathologica Communications | Full Text

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By iPSC Laboratory, Laboratorio di Neurogenetica, Dipartimento di Neuroscienze
| Biomed Central (BMC)
All materials were purchased from ThermoFisher Scientific, unless specified. The generation and characterization of human iPSC lines CSC-37N (healthy control aged 56-year-old) and CSC-17F (APP p.V717I, heterozygous, aged 50-year-old) has been previously reported [7]. After several passages in vitro, iPSCs were differentiated into hippocampal spheroids as previously described [7].
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Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

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By Jeovanis Gil, Yuriy Pomeshchik, iPSC Laboratory, Oxana Klementieva
| Stem Cell Reports Verified
Highlights • Rapid generation of hippocampal spheroids (HSs) from hiPSCs using defined chemical agents • hiPSC-derived HSs can be utilized as a source of hippocampal neurons • hiPSC-derived HSs can be used to model Alzheimer disease • hiPSC-derived HSs can be used to develop innovative therapeutic solutions Summary The hippocampus is important for memory formation and is severely affected in the brain with Alzheimer disease (AD).
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