Iro K. Ventouri on Muck Rack

Iro K. Ventouri

As seen in: ACS Publications
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Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

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By Iro K. Ventouri, Sander Kok, Maarten Honing, Govert W. Somsen
| ACS Publications
Size-exclusion chromatography employing aqueous mobile phases with volatile salts at neutral pH combined with electrospray-ionization mass spectrometry (SEC-ESI-MS) is a useful tool to study proteins in their native state. However, whether the applied eluent conditions actually prevent protein–stationary phase interactions, and/or protein denaturation, often is not assessed.
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