Request a detailed protocol For imaging, 0.1–0.5 ng of mRNA encoding Dvl2-mCh, Dvl2-mSc, Dvl2-EGFP, Fz7-EGFP, XRor2-tdTomato, XRor2-EGFP, EGFP-Vangl2, EGFP-Vangl2 RH, flag-XPk, EGFP-mPk2, XWnt11, mem-GFP, and mem-mCh were injected in various combinations into the animal regions at the two-cell stage and dissected at ~St.9, or the DMZ at the four-cell stage and dissected at St. 10.25.