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Izak F. Prinsloo on Muck Rack

Izak F. Prinsloo

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The evaluation of isatin analogues as inhibitors of monoamine oxidase

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By Izak F. Prinsloo, Jacobus P. Petzer, Theunis T. Cloete, Anél Petzer
| Wiley Online Library Verified
The determination of IC50 values for MAO inhibition Recombinant human MAO-A and MAO-B were obtained from Sigma-Aldrich, and IC50 values were measured and calculated as described in literature (Mostert et al., 2015; Weissbach et al., 1960). Kynuramine was used as substrate for both MAO isoforms. MAO oxidizes kynuramine to produce 4-hydroxyquinoline. Since 4-hydroxyquinoline fluoresces in a basic pH, its formation can be monitored using fluorescence spectrophotometry.
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