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Thirty male C57BL/6J mice and thirty male Apoe-/- mice were purchased from the Huafucang Biotechnology Co., Ltd. (Beijing, China) with animal license SCXK 2019-0008. All mice, in cages of five, were housed in a standard SPF environment, specifically 20−25 °C room temperature, 40–70% humidity and free access to ultrafiltered water, with the exception of mice requiring chronic stress interventions that were on a 12h/12 h day and night cycle (Hu et al., 2022).
Discussion The findings of this study reveal the salivary proteome during HM stress. After 2 h, HM stress suppressed salivary antimicrobial proteins such as KLK1, CST4, and IGK in association with IGHA1. After 4 h, HM, IGHA1, KLK1, and CST4, but not IGK, were suppressed after 2 h post-HM. In addition, the relative abundances of IGHA1, CST4, and IGK were positively correlated 2 h and 4 h post-HM.
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