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Flanking sequences together with the core EEF1A1 promoter into the expression plasmid might be useful due to the positive regulation of TFBS and the genetic elements, which prevented the heterochromatinization of the insert region. The first intron of the Chinese hamster EEF1A1 gene was 62% identical to the orthologous human intron (Wakabayashi-Ito & Nagata, 1994), and contained numerous potential TFBS (Boulikas, 1994).
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