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Articles

Combinatorial CRISPR screens and lectin microarrays identify novel glycosylation regulators

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By C Kimberly Tsui, Nicholas Twells, Emma Doan, Janiya Brooks
| biorxiv.org
Abstract Glycans play critical roles in cellular signaling and function. Unlike proteins, glycan structures are not templated from genes but the concerted activity of many genes, making them historically challenging to study. Here, we present a strategy that pairs pooled CRISPR screens with lectin microarrays to uncover and characterize regulators of cell surface glycosylation.
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