Jeroen Sidney Dickschat

A clickable coenzyme A derived probe for investigating phosphopantetheinyl transferase activity in natural product biosynthesis adjust margins Phosphopantetheinyl transferases (PPTases) activate carrier proteins (CPs) through attachment of a coenzyme A (HSCoA) derived phosphopantetheinyl linker. This study describes a method to monitor this process through a modified HSCoA with an alkyne group, allowing for the Cu-catalysed alkyne-azide cycloaddition of a fluorescent tag.

Mechanistic Characterisation of the Diterpene Synthase for Clitopilene and Identification of Isopentalenene Synthase from the fungus Clitopilus passeckerianus Two terpene synthases from the pleuromutilin producing fungus Clitopilus passeckerianus were functionally characterised. The first enzyme CpTS1 produces the new diterpene clitopilene with a novel 6-6-5-5 tetracyclic skeleton, while the second enzyme CpTS2 makes the new sesquiterpene isopentalenene.

Mechanistic Characterisation of a Sesquiterpene Synthase for Asterisca-1,6-diene from the Liverwort Radula lindenbergiana and Implications for Pentalenene Biosynthesis A sesquiterpene synthase from the liverwort Radula lindenbergiana was characterised and shown to produce the new sesquiterpene hydrocarbon (3R,9R)-asterisca-1,6-diene, besides small amounts of pentalenene.