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Abstract DNA methylation is an important epigenetic mechanism essential for transposon silencing and genome integrity. Across evolution, the substrates of DNA methylation have diversified between kingdoms to account for genome complexity. In plants, Chromomethylase3 (CMT3) and CMT2 are the major methyltransferases mediating CHG and CHH methylation, respectively. However, how these two enzymes diverge on substrate specificities during evolution remains unknown.
Author notes These authors jointly supervised this work: Robert T. Manguso, Russell W. Jenkins Authors and Affiliations Massachusetts General Hospital Cancer Center, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA Yi Sun, Or-yam Revach, Amina Fu, Xiang Ma, Jia Gwee, Princy Sindurakar, Jun Tian, Arnav Mehta, Moshe Sade-Feldman, Thomas LaSalle, Hongyan Xie, Rodrigo Saad-Beretta, Kathleen B. Yates, Angelina M. Cicerchia, Martin Q. Rasmussen, Samuel J.
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