Jiaxiu Yin

Abstract Background: Male testis is highly susceptible to irreversible damage from acute myeloid leukemia (AML) infiltration and subsequent chemotherapy. As a gene critical for sperm function, the role of SPAG6 in AML testicular infiltration and chemotherapy-induced injury remains poorly elucidated. Methods: SPAG6 expression was knocked down in C1498 leukemia cell line, and its impact on testicular infiltration was evaluated in vivo. RNA sequencing was performed to identify altered pathways.

1 Introduction Acute myeloid leukemia (AML) is a profoundly heterogeneous malignant disorder with substantial genetic heterogeneity and clonal variation, resulting in a high mortality rate and poor prognosis [1, 2]. The accumulation of poorly differentiated myeloid cells contributes to the pathological features of AML, including anemia, bleeding, infection, and death [3].