Abstract To be able to trap and image in a live cell/organism on the go is an incredible feat and paves the way for immobilization-free interrogation. This is a step towards the interrogation of cells/live species in their natural environment. To facilitate, a TRIMing technique primarily based on an adaptive lightsheet optical tweezer (aLOT) system is proposed. The TRIMing technique combines the benefits of touch-free optical tweezing and high-resolution imaging.