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Neptune Baro

As seen in: BioRxiv
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Articles

TRapping and IMaging (TRIMing) of Cells/Multicellular Organisms in Free Living Environment Enabled by Adaptive Lightsheet Optical Tweezer (aLOT)

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By Neptune Baro, Neeraj Pant, Partha Mondal, Jigmi Basumatary
| BioRxiv
Abstract To be able to trap and image in a live cell/organism on the go is an incredible feat and paves the way for immobilization-free interrogation. This is a step towards the interrogation of cells/live species in their natural environment. To facilitate, a TRIMing technique primarily based on an adaptive lightsheet optical tweezer (aLOT) system is proposed. The TRIMing technique combines the benefits of touch-free optical tweezing and high-resolution imaging.
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Planar Optical Tweezer Trap (2D-LOT) System Realized by Light Sheet Illumination & Orthogonal Widefield Detection

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By Partha Mondal, Neptune Baro
| BioRxiv
Abstract We report the realization of the first planar optical tweezer trap system by a sheet of light. To visualize the trapping of the target object (dielectric bead or live cell) in a plane, an orthogonal widefield detection is employed. The planar / two-dimensional lightsheet optical tweezer (2D-LOT) sub-system is realized in an inverted microscopy mode with illumination from the bottom.
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