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Joaquin de Lapuente

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Measuring DNA modifications with the comet assay: a compendium of protocols - Nature Protocols

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By Peter Rask Møller, Goran Gajski, Elisa Boutet-Robinet, Marcus S. Cooke, Susan Duthie, Óscar Herrero Verified, Awadhesh Jha, Gudrun Koppen, Ricard Marcos, Mirta Milić, Massimo Moretti, Rui F. Oliveira, Ann-Karin Olsen, Helga Stopper, João Teixeira, Mahara Valverde, Tomas Zikmund, Joaquin de Lapuente, Marina Isidori, Marcin Kruszewski, Blanca Laffon, Ludovic Le Hégarat, Angélique Lewies, Julen Sanz-Serrano, Vanessa Valdiglesias, Johannes F. Wentzel, Maciej Wnuk, Bojana Žegura
| Nature Verified
Abstract The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA–DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts.
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