Citation: Mantlo E, Maruyama J, Manning JT, Reyna RA, Huang C, Paessler S (2025) Development and characterization of a reverse genetics system for the lineage II Chicava strain of Machupo virus in a guinea pig model. PLoS Negl Trop Dis 19(1): e0012834. https://doi.org/10.1371/journal.pntd.0012834 Editor: Ran Wang, Beijing Children’s Hospital Capital Medical University, CHINA Received: September 29, 2024; Accepted: January 10, 2025; Published: January 24, 2025 Copyright: © 2025 Mantlo et al.