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AVHANDLINGAR.SE: How evolutionary adaptation perfects the pathogenic lifestyle : structural characterization of pathogenic protein complexes, machineries, and virulence factors
Nyckelord: NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microsporidia; Vibrio cholerae; Evolutionary adaptation; Mechanosensitive ion channel of small conductance; Alpha pore-forming toxins; Single particle cryo-electron microscopy; Genome annotation; Biochemistry; biokemi; Infectious Diseases; infektionssjukdomar; molekylärbiologi; Molecular Biology; Sammanfattning: Pathogens have developed sophisticated strategies to thrive in hostile host environments.
Nematocida displodere Mechanosensitive Ion Channel of Small Conductance 2 assembles into a unique 6-channel super-structure in vitro
Abstract Mechanosensitive ion channels play an essential role in reacting to environmental signals and sustaining cell integrity by facilitating ion flux across membranes. For obligate intracellular pathogens like microsporidia, adapting to changes in host environment is crucial for survival and propagation.
Functional annotation of a divergent genome using sequence and structure-based similarity - BMC Genomics
V. necatrix spores were propagated in the fourth and fifth instar larvae of Helicoverpa zea (corn earworm). The larvae were homogenized in Fisher 50 mL closed Tissue Grinder System tubes in water, filtered through a double layer of cheesecloth, and further filtered through 100 and 40 μm Biologix centrifugal filters before storage at − 80 ˚C until further use. For genomic DNA extraction, V.
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