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Josie Schultz

As seen in: Nature, BioRxiv

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Articles

Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster

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By Natalie L Venette-Smith, Rishi K Vishwakarma, Varun Venkatakrishnan, Roberta Dollinger, Josie Schultz, Paul Babitzke, Ganesh S. Anand, David Gilmour, Jean-Paul Armache, Katsuhiko S. Murakami
| Nature Verified
Abstract Structural studies of eukaryotic RNA polymerase II (Pol II) transcription often rely on in vitro assembly, which may not fully represent native conditions. To investigate Pol II transcription in metazoan cells, we developed a method to isolate native transcription complexes from Drosophila melanogaster embryos using FLAG-tag affinity purification and Micrococcal Nuclease treatment.
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Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster

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By Rishi K Vishwakarma, Roberta Dollinger, Josie Schultz, Natalie L Venette-Smith
| BioRxiv
Abstract Structural studies of eukaryotic RNA polymerase II (Pol II) transcription complexes often depend on in vitro assembly by mixing purified Pol II with synthetic DNA/RNA scaffolds, recombinant transcription factors, and/or histones, followed by stalling transcription at defined positions by adding selected nucleotide triphosphate substrates.
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