Julia Järlebark

Introduction Click to copy section linkSection link copied! Nanopores have emerged as an important sensor technology for single biomolecule detection and analysis. With the exception of optical detection, the transduction mechanism is generally based on changes in the ionic conductance of a single pore when molecules are present inside, (1,2) in analogy with the Coulter counter for detecting cells in microscale capillaries. The nanopores can be either solid state, biological, or hybrid variants.

Introduction Click to copy section linkSection link copied! Nanopores have emerged as an important sensor technology for single biomolecule detection and analysis. With the exception of optical detection, the transduction mechanism is generally based on changes in the ionic conductance of a single pore when molecules are present inside, (1,2) in analogy with the Coulter counter for detecting cells in microscale capillaries. The nanopores can be either solid state, biological, or hybrid variants.

Abstract The possibility to detect and analyze single or few biological molecules is very important for understanding interactions and reaction mechanisms. Ideally, the molecules should be confined to a nanoscale volume so that the observation time by optical methods can be extended. However, it has proven difficult to develop reliable, non-invasive trapping techniques for biomolecules under physiological conditions.