Julie Carnesecchi

Abstract Protein interaction networks (or interactomes) are formed progressively, each interaction influencing the next one. Accordingly, a same protein will establish different interactomes depending on its first associated cofactor, thereby diversifying its function in the cell. In contrast to their central role, few methods exist to capture interactomes of dimeric protein complexes.

Abstract Protein function depends on interactions with other protein partners, ultimately leading to the formation of intricate protein-protein interaction (PPI) networks. These molecular networks (or interactomes) are formed progressively, each interaction influencing the next one. Accordingly, a same protein can lead to the formation of different interactomes depending on its first associated cofactor.

7 CONCLUSION AND FUTURE DIRECTIONS TFs are pivotal in integrating transcription and splicing, acting at both levels through interconnected mechanisms. Nonetheless, we are still far from understanding their molecular mode of action. Several models have been proposed yet, most of them remain to be experimentally tested and connected. This is where the importance of multidisciplinary research strategies stands.