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Julie Van De Velde on Muck Rack

Julie Van De Velde

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Articles

A laser capture microdissection-based method for high-sensitivity transcriptomics from archived FFPE tissue slides with single-cell resolution using LCM-FFPEseq

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By Hannes Syryn, Koen Deserranno, Danique Berrevoet, Dieter Deforce, Ward De Spiegelaere, Koen Van de Vijver, Martine Cools, Filip Van Nieuwerburgh, Elise Callens, Guillaume Cattebeke, Henri Couckuyt, Julie Van De Velde, Aaron Verplancke
| Nature Verified
Abstract Understanding gene expression within its spatial context is essential for unravelling biological processes. Laser capture microdissection (LCM) enables targeted isolation of cells or regions from tissue sections while preserving spatial context. LCM-based RNA sequencing is predominantly applied to fresh-frozen tissue, and for formalin-fixed paraffin-embedded (FFPE) material, most workflows rely on commercial kits requiring high input quantities, limiting their use for rare cells.
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