Kairang Jin on Muck Rack

Kairang Jin

As seen in: Stem Cell Reports
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Articles

Shugoshin 2A stabilizes heterochromatin complexes to suppress the 2-cell-like state in embryonic stem cells

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By Panpan Shi, Guoxing Yin, Lin Liu, Kairang Jin
| Stem Cell Reports Verified
Methods E3 and J1 cell lines were used in this study. E3 ESCs were cultured on MEF feeder cells (Ye et al., 2021), whereas J1 ESCs were cultured on gelatin. The culture medium of ESCs consisted of 15% fetal bovine serum (ES quality, HyClone) supplemented with penicillin (100 U/mL) and streptomycin (100 μg/mL), LIF (1000 U/mL) (ESGRO, Chemicon), nonessential amino acids, β-mercaptoethanol (0.1 mM), L-glutamine (1 mM), and knockout DMEM (Gibco).
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