Kamalraj Devaraj

We have described the ability of newly synthesized fluorescent probe (LP1) for detecting phosphatase activity in lysosomes in cancer cells. Probe LP1 displayed 33-fold fluorescence intensity enhancement at λem 532 nm in the presence of phosphatase in HEPES buffer (pH 4.5). The quantum yield of probe LP1 was increased ~21- fold upon exposure to phosphatase in acidic pH. The probe LP1 is highly chemoselective toward phosphatase and free from interference by ubiquitous biological analytes.