Katja Schroeder

Abstract Zebrafish (Danio rerio) is widely used as a model organism in biological research. However, the impact of different incubation temperatures on specific developmental endpoints is still insufficiently studied. This study quantifies developmental differences of zebrafish embryos incubated at two commonly used temperatures (26 degrees and 28 degrees), focusing on key developmental endpoints (onset of heartbeat, hatching time, eye size, yolk sac consumption, body length).

Abstract Zebrafish (Danio rerio) early development stages that do not feed independently, are classified as non-protected life stages under EU Directive 2010/63. Zebrafish reach the independently feeding stage not earlier than 120 hours post fertilization, depending on the incubation temperature. This paper presents a dataset documenting zebrafish early development at two commonly used temperatures 26 and 28 degrees Celsius.