Kellie Johnson

Cookies, device or similar online identifiers (e.g. login-based identifiers, randomly assigned identifiers, network based identifiers) together with other information (e.g. browser type and information, language, screen size, supported technologies etc.) can be stored or read on your device to recognise it each time it connects to an app or to a website, for one or several of the purposes presented here.

The use of immunoassays for the quantification of analytes in biological samples such as, plasma, serum, tissue homogenates, etc. can be challenging. There are many components in these samples, such as carbohydrates, proteins, and phospholipids that can interfere with the ability of the antibody pairs to bind to their target. This phenomenon is known as the "Matrix Effect". How do you know that you have entered the "Matrix"?

Traditional sandwich ELISAs and bead-based multiplex immunoassays, such as LEGENDplex™, are frequently used to detect and quantify specific analytes within a biological sample. These samples include serum, plasma, cell culture supernatants, and other biological matrices. In order to determine the concentration of an analyte within a sample, one must run a standard, or calibration, curve. The production of a standard curve requires the use of known concentrations of the analyte being assayed.