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Improved vector toolkit for genome writing in mammalian cells
ABSTRACT Efficient genome writing in mammalian cells requires robust methods for integrating large DNA payloads. The previously described method mammalian Switching Antibiotic resistance markers Progressively for Integration (mSwAP-In) enables iterative, biallelic genome rewriting in mammalian stem cells with DNA payloads exceeding 100 kb. However, the lack of standardized vectors and certain technical constraints have limited its broader adoption.
Transmission, localization, and infectivity of seedborne maize chlorotic mottle virus
Statistical analyses When insufficient tissue was available for re-testing each individual plant in an MCMV positive pool, estimates of MCMV seed transmission (p) frequencies were calculated as: where Q is the proportion of positive pooled samples and n is the number of plants in each pool [44]. Upper and lower bounds were calculated at a 95% confidence level based on the Wilson score interval for these positive seed lots [45].
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