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Khai Khee Kho on Muck Rack

Khai Khee Kho

As seen in: BioRxiv
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Articles

Combinatorial mutagenesis of N-terminal sequences reveals unexpected and expanded stability determinants of the Escherichia coli N-degron pathway

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By Sabyasachi Sen, Nastassja Corrado, Alexander Tiso, Khai Khee Kho
| BioRxiv
Abstract Although it is known that residues near the N-terminus can influence protein stability, there has not been a comprehensive effort to document how these penultimate residues impact protein stability in prokaryotes. Here, we utilize combinatorial mutagenesis, cell sorting, and next generation sequencing to perform the deepest sequence coverage screen of the bacterial N-degron pathway of proteolysis.
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Combinatorial mutagenesis of N-terminal sequences reveals unexpected and expanded stability determinants of the Escherichia coli N-degron pathway

|
By Sabyasachi Sen, Nastassja Corrado, Alexander Tiso, Khai Khee Kho
| BioRxiv
Abstract Although it is known that penultimate N-terminal residues can influence protein stability by the N-degron pathway, there has not been a comprehensive effort to document nor predict these effects for natural and synthetic sequences in prokaryotes. Here, we present the deepest sequence coverage screen of the bacterial N-degron pathway using combinatorial site-saturation mutagenesis, fluorescence-activated cell sorting (FACS), and next-generation sequencing (NGS).
Open in Who Shared Issue with article?
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