Kieop Park

Abstract To breach basement membrane (BM), cells in development and cancer use large, transient, specialized lipid-rich membrane protrusions. Using live imaging, endogenous protein tagging, and cell-specific RNAi during C. elegans anchor cell (AC) invasion, we demonstrate that the lipogenic SREBP transcription factor SBP-1 drives expression of the fatty acid synthesis enzymes POD-2 and FASN-1 prior to invasion.

Stored inside the fat deposits of humans and mice are thermogenic cells known as brown and beige adipocytes which burn energy and dissipate it as heat (Sakers et al., 2022). This allows the body to stay warm when temperatures drop and to maintain energy levels in response to physical activity. As individuals age, their ability to carry out this function – known as adaptive thermogenesis – declines, but it remains unclear why.

C. elegans culture and strains Request a detailed protocol C. elegans strains used in this study are listed in the Key resources table. All newly generated strains and associated plasmids and primers (source label ‘this study’ in the Key resources table) are available upon request. Worms were reared on nematode growth medium plates seeded with OP50 Escherichia coli at 16°C, 18°C, or 20°C according to standard procedures (Stiernagle, 2006). We used C.