Kim Bartels

Abstract The predominant resistance mechanism observed in Gram-negative bacteria involves the production of β-lactamases, which catalyse the hydrolysis of β-lactam antibiotics, thereby rendering them ineffective. Although isoxazolyl penicillins are available since the 1970s, there are currently no structures in complex with class-A β-lactamases available.

Main text (Cell Reports Physical Science 5, 101987; June 19, 2024) In the original version of this article, Tables 2–6 should have included PDB-IDs. These were accidentally excluded due to an error during LaTeX conversion and typesetting, which was not caught during proofing. The article has now been updated online, and the corrected tables also appear below. The authors apologize for the error.

Abstract Photon-by-photon analysis tools for diffusion-based single-molecule Foerster resonance energy transfer (smFRET) experiments often describe protein dynamics with Markov models. However, FRET efficiencies are only projections of the conformational space such that the measured dynamics can appear non-Markovian. Model-free methods to quantify FRET efficiency fluctuations would be desirable in this case. Here, we present such an approach.